Bursaria truncatella Müller 1773

Most likely ID: n.a.

 

Synonym: n.a.

 

Sampling location: Simmelried, Purren pond, Mainau pond

 

Phylogenetic tree: Bursaria truncatella

 

Diagnosis: 

  • body broadly pouch-shaped to reniform,
  • length 200–1700 µm (commonly 300–600 µm)
  • mouth opening the entire front end, with deep ventral incision
  • cytoplasm strongly vacuolated
  • macronucleus vermiform, length 1000–1500 µm
  • 140–250 longitudinal rows of paired cilia
  • micronuclei spherical (3–5 µm), about 10–120 scattered in cytoplasm
  • about 300–500 contractile vacuoles beneath cortex
  • extrusomes (mucoscysts) 1–3 µm long
Bursaria-truncatatella
Bursaria truncatatella

Bursaria truncatella is one of the largest ciliates that I rarely, but regularly find in some of my sampling sites. In the samples, the specimens usually swim just above the mud layer at the bottom and can be seen with the naked eye.

 

The oral apparatus of Bursaria truncatella is very complicated designed and difficult to examine in living specimens. Dominant is the adoral zone of membranelles, which begins on the left side of the ventral incision and takes a U-shaped course (s. fig. 2 and 5). Shortly before the posterior end it bends back and ends in the anterior third (s. fig. 2). Bursaria truncatella is omnivorous and can also devour very large prey. Numerous eaten ciliates and colonies of algae are often found. But rotifers are also among the prey organisms.

 

The macronucleus is vermiform and, according to my observations, lies parallel to the adoral zone of membranelles in unsquashed specimens. In squashed specimens it detaches from the oral tube and becomes entangled (s. fig. 6).

 

Numerous micronuclei are scattered in the cytoplasm (s. fig. 7), but they are difficult to detect in the highly vacuolated cytoplasm and between the food vacuoles. In addition to these micronuclei scattered in the cytoplasm, I was also able to find micronuclei attached to the macronucleus (s. fig. 8). These micronuclei adjacent to the macronucleus are not mentioned in the literature.

Bursaria-truncatatella

Fig. 1 a-b: Bursaria truncatella. L = 930 µm. A freely swimming (a) and slightly squashed specimen (b). Obj. 4 X.

Bursaria-truncatatella

Fig. 2: Bursaria truncatella. L = 930 µm. Focal plane on the U-shaped adoral zone of membranelles (arrows) of the specimen as shown in fig. 1 a-b. Obj. 20 X.

Bursaria-truncatatella

Fig. 3 a-b: Bursaria truncatella. L = 680 µm. A second freely swimming specimen with focal plane on the ventral incision (VI) of the mouth opening. Obj. 20 X.

Bursaria-truncatatella

Fig. 4: Bursaria truncatella. Detail of the ventral incision. Note the dense ciliation. Obj. 40 X.

Bursaria-truncatatella

Fig. 5: Bursaria truncatella. Detail of the central incision with focal plane on the adoral zone of membranelles (AZM) at the left side. Obj. 40 X.

Bursaria-truncatatella

Fig. 6: Bursaria truncatella. The vermiform macronucleus (Ma) in a squashed specimen. Obj. 40 X.

Bursaria-truncatatella

Fig. 7: Bursaria truncatella. Two of the numerous micronuclei (Mi) scattered in the cytoplasm. Obj. 60 X.

Bursaria-truncatatella

Fig. 8: Bursaria truncatella. Some micronuclei (arrows) are adjacent to the macronucleus. Obj. 40 X.

Bursaria-truncatatella

Fig. 9: Bursaria truncatella. Two of the numerous contractile vacuoles (CV) scattered over the cortex and an exctretion porus (EP). Obj. 100 X.

Bursaria-truncatatella

Fig. 10: Bursaria truncatella. The paired cilia of the longitudinal rows of cilia. Obj. 100 X.

Bursaria-truncatatella

Fig. 11: Bursaria truncatella. Two specimens in conjugation. Obj. 20 X.