two chloroplasts, each with 3-10 longitudinal ridges
numerous pyrenoids scattered in the chloroplasts
girdle bands absent
apices with each one vacuole filled with numerous gipsum crystals
cell wall with fine striation (12-20 striae/10 µm)
nucleus central
Closterium ehrenbergii is a very common representative of the genus Closterium. In addition to the impressive size of usually over 400 µm, the pyrenoids scattered in the chloroplasts are the main identifying feature (s. figs. 2 and 3). In all other species of Closterium, except Closterium lunula, the pyrenoids are arranged more or less along the longitudinal cell axis. The cell shape of Closterium lunula, however, is almost straight and less curved.
The cell wall of Closterium ehrenbergii has a very fine striation (s. fig. 4). This can be more or less pronounced. In the older literature, the subspecies Closterium ehrenbergii var. malinvernianum is defined as having a more pronounced striation. However, Lenzenweger (1996) considers this to be an uncertain characteristic, which is why the delimitation of a subspecies does not appear to be justified. Although the specimens of my population showed a fine but clearly visible striation, I stick to the identification Closterium ehrenbergii.
Fig. 1 a-b:Closterium ehrenbergii. L = 425 µm. Two focal planes of a specimen in brightfield illumination. Obj. 40 X.
Fig. 2:Closterium ehrenbergii. L = 425 µm. The same specimen as shown in fig. 1 a-b in DIC. Note the pyrenoids (PY) scattered in the chloroplasts. Obj. 40 X.
Fig. 3:Closterium ehrenbergii. The pyrenoids (PY) scatteres in the chloroplasts in detail. Beside the pyrenoids some crystals (CR) are visible. Obj. 100 X.
Fig. 4:Closterium ehrenbergii. Focal plane on the fine striattion of the cell wall. In this specimen 17 striae/10 µm are present. Obj. 100 X.
Fig. 5:Closterium ehrenbergii. The rounded apex with numerous crystales in the terminal vacuole. Obj. 100 X.