Diplophrys archeri Barker, 1868

Most likely ID: n.a.

 

Synonym: n.a.

 

Sampling location: Simmelried

 

Phylogenetic tree: Diplophrys archeri

 

Diagnosis: 

  • cells spherical or elliptical, with thin membrane
  • two pseudostomes at opposite poles without neck
  • diameter 8–20 µm
  • pseudopodia straight or dichotomously branched
  • several, peripher contractile vacuoles
  • spherical nucleus with a central nucleolus
  • one or several large oily droplets in cytoplasm
  • oily droplet colorless, yellowish, orange or red
  • can form colonies after several cell divisions
Diplophrys-archeri
Diplophrys archeri

I find Diplophrys archeri frequently and regularly in the Simmelried. Especially in old samples with little plant material, the specimens can be found at the bottom of the container.

 

In my population almost all specimens had only an oil droplet of orange color. Only rarely was the oil colorless. The pseudopodia are often completely retracted under the coverslip. Therefore, the specimens should be placed under the coverslip with a high layer thickness and then allow to evaporate slowly. In rare cases Diplophrys archeri settles also on the floating coverslip.

 

The cells are surrounded by a thin, colorless membrane, which forms a soft test. This membrane is difficult to see, but in some specimens it is thicker (about 1.0–1.2 µm) and then easier to recognize (s. fig. 2).

 

Sometimes specimens in cell division can be found (s. fig. 4 a-b). Depending on how often the mother cell divides, stages with 2, 4, or 8 cells can be found. If cell division continues to occur more frequently, spherical clusters of daughter cells form, which initially have a diameter of only 4–5 µm.

Diplophrys-archeri

Fig. 1: Diplophrys archeri. D = 17.9 µm. A specimen with widely spreaded, branched pseudopodia. Nu = nucleus. Obj. 100 X.

Diplophrys-archeri

Fig. 2 a-b: Diplophrys archeri. D = 11 µm. Two focal planes of a smaller specimen. CV = contractile vacuole, Nu = nucleus. Obj. 100 X.

Diplophrys-archeri

Fig. 3: Diplophrys archeri. D = 15.6 µm. A second specimen with spreaded pseudopodia. Note the hyaline membrane (HM) covering the cell. Obj. 60 X.

Diplophrys-archeri

Fig. 4 a-b: Diplophrys archeri. Two specimens in the process of cell division. CV = contractile vacuole, Nu = nucleus. Obj. 100 X.